Publications
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Luan, S., Kudla, J., Manuel Rodriguez-Concepcion, M., Yalovsky, S. and Gruissem W. CaMs and CBLs: Calcium Sensors for Specific Signal-Response Coupling in Plants. The Plant Cell, Vol 14 Supplement, 2002 (pp. S389-400).
Many extracellular signals, including light, biotic, and abiotic stress factors, elicit changes in cellular Ca2 concentration in plants...
Bracha-Drori, K., Lavy, M. and Yalovsky, S. The Arabidopsis AtSTE24 is a CaaX Protease with Broad Substrate Specificity. Journal of Biological Chemistry, Vol 277, 2002
Following prenylation, the proteins are subject to two prenyl-dependent modifications at their C-terminal end, which are required for their subcellular targeting...
Lavy, M., Bracha-Drori, K., Sternberg, H. and Yalovsky, S. Cell-specific prenylation independent mechanism regulates targeting of type-II RACs. The Plant Cell, Vol 14, 2002 (pp. 2431-2450)
The RHO proteins, which regulate numerous signaling cascades, undergo prenylation, facilitating their interaction with membranes and with proteins called RHO·GDP dissociation inhibitors..
Mark P. Running, M.P. Lavy, M., Sternberg, H., Galichet, A., Gruissem, W., Hake, S., Ori, N. and Yalovsky, S., Enlarged meristems and delayed growth in plp mutants result from lack of CaaX prenyltransferases. Proceedings of the National Academy of Sciences USA, Vol 101, 2004 (pp. 7815-7820)
Meristems require a myriad of intercellular signaling pathways for coordination of cell division within and between functional zones and clonal cell layers...
Bracha-Drori, K., Shichrur, K., Katz, A., Oliva, M., Angelovici, R., Yalovsky, S. and Ohad, N., Detection of protein-protein interaction in plants using YFP fluorescence complementation. The Plant Journal, Vol 40, 2004 (pp. 419-427)
Protein function is often mediated via formation of stable or transient complexes...
Bloch, D., Lavy, M., Efrat, Y., Efroni, I., Bracha-Drori, K., Abu-Abied, M., Sadot, E. and Yalovsky, S., Ectopic expression of an activated RAC in Arabidopsis disrupts membrane cycling. Molecular Biology of the Cell, Vol 16, 2005 (pp. 1913-1927).
Rho GTPases regulate the actin cytoskeleton, exocytosis, endocytosis, and other signaling cascades...
Lavy, M and Yalovsky, S., Association of Arabidopsis type-II ROPs with the plasma membrane requires a conserved C-terminal sequence motif and a proximal polybasic domain. The Plant Journal, Vol 46, 2006 (pp. 934-947).
Plant ROPs (or RACs) are soluble Ras-related small GTPases that are attached to cell membranes by virtue ofthe post-translational lipid modifications of prenylation and S-acylation...
Liu, C., Zhou, J. Bracha-Drori, K., Yalovsky, S., Ito, T. and Yu, H., Specification of Arabidopsis floral meristem identity by repressing flowering time genes. Development, Vol 134, 2007 (pp. 1901-1910).
Flowering plants produce floral meristems in response to intrinsic and extrinsic flowering inductive signals...
Lavy, M., Bloch, D., Hasak, O., Gutman, I., Poraty, L., Sorek, N., Sternberg, H. and Yalovsky, S., A novel ROP/RAC effector links cell polarity root meristem maintenance and vesicle trafficking. Current Biology, Vol 17, 2007 (pp. 947-952).
ROP/RAC GTPases are master regulators of cell polarity in plants...
Ohad, N., Shichrur, K., and Yalovsky, S., The analysis of protein-protein interactions in plants by Bimolecular Fluorescence Complementation (BiFC). Plant Physiology, Vol 145, 2007 (pp.1090-1099).
Following the complete genome sequencing of different plant species such as Arabidopsis (Arabidopsis thaliana), rice (Oryza sativa), and Physcomitrella (Physcomitrella patens), as well as advances toward deciphering entire proteomes, the need for a reliable way to identify protein-protein interactions is becoming a major task for the future...
Baerenfaller, K., Grossmann, J., Grobei, M. A. Hull, R., Hirsch-Hoffmann, M., Yalovsky, S., Zimmermann, P., Grossniklaus, U., Gruissem, W. and Baginsky, S., Genome-scale proteomics reveals new Arabidopsis thaliana gene models and proteome dynamics. Science, Vol 320, 2008 (pp. 938-941).
We have assembled a proteome map forArabidopsis thalianafrom high-density...
Batistič, O., Sorek, N., Schültke, S., Yalovsky, S. and Kudla, J., Dual fatty acyl modification determines the localization and plasma membrane targeting of CBL/CIPK Ca2+ signaling complexes. The Plant Cell, Vol 20, 2008, (pp. 1346-1362).
Arabidopsis thaliana calcineurin B–like proteins...
Bracha-Drori, K., Shichrur, K., Cohen Lubetzky, T. and Yalovsky, S., Functional analysis of Arabidopsis post-prenylation CaaX processing enzymes and their function in subcellular protein targeting. Plant Physiology, Vol 148, 2008, (pp. 119-131).
Prenylation is a posttranslational protein modification...
Yalovsky, S., Bloch, D., Sorek, N. and Kost, B., Regulation of membrane trafficking, cytoskeleton dynamics and cell polarity by ROP/RAC GTPases. Plant Physiology, Vol 147, 2008, (pp. 1527-1543).
Prenylation is a posttranslational protein modification essential for developmental processes and response to abscisic acid...
Sorek, N., Bloch, D. and Yalovsky, S., Protein lipid modifications in signaling and subcellular targeting. Current Opinion in Plant Biology, Vol 12, 2009 (pp. 714-720).
Classically perceived as means for recruiting proteins to the membranes...
Hazak, O., Bloch, D., Poraty, L., Sternberg, H., Zhang, J., Friml, J. and Yalovsky, S., A rho scaffold integrates the secretory system with feedback mechanisms in regulation of auxin distribution. PLoS Biology, Vol 2010 (pp. e1000282).
Development in multicellular organisms depends on the ability...
Sorek, N. and Yalovsky, S., Analysis of protein S-acylation by gas chromatography–coupled mass spectrometry using purified proteins. Nature Protocols, 2010, Vol 5.
S-acylation, also known as palmitoylation, involves the attachment of acyl fatty acids to thiol...
Sorek, N., Segev, O., Gutman, O., Bar, E., Richter, S., Poraty, L., Hirsch, J. A., Henis, Y. I., Lewinsohn, E., Jürgens, G. and Yalovsky, S., An S-acylation switch of conserved G-domain cysteines is required for polarity signaling by ROP GTPases. Current Biology, Vol 20, 2010, (pp. 914-920).
Rho GTPases are master regulators of cell polarity...
Bloch, D., Monshausen, G., Singer, M., Gilroy, S. and Yalovsky, S., Nitrogen source interacts with ROP signaling in root hair tip-growth. Plant Cell and Environment, Vol 34, 2011, (pp. 76-88).
Root hairs elongate in a highly polarized manner known astip growth.
Sorek, N. Gutman, O., Bar, E., Abu-Abied, M., Feng, X., Mark P. Running, M. P., Lewinsohn, E., Ori, N., Sadot, E., Henis, Y. I. and Shaul Yalovsky, S., Differential effects of prenylation and S-acylation on type-I and II ROPs membrane interaction and function. Plant Physiology, Vol 155, 2011, (pp. 706-720).
Prenylation primarily by geranylgeranylation is required for membrane attachment and function of type I Rho of Plants (ROPs) and Gg proteins, while type II ROPs are attached to the plasma membrane by S-acylation...
Wu, H-M., Hazak, O., Cheung, A. Y. and Yalovsky, S. RAC/ROP GTPases and auxin signaling. The Plant Cell, Vol 23, 2011 (pp. 1208-1218).
Auxin functions as a key morphogen in regulating plant growth and development...
Batistič, O., Rehers, M. Akerman, A., Steinhorst, L., Schlücking, K. Yalovsky, S. and Kudla, J.. S-acylation dependent association of the calcium sensor CBL2 with the vacuolar membrane is essential for proper abscisic acid responses. Cell Research, Vol 22, 2012 (pp. 1155-1168).
Calcineurin B-like (CBL) proteins contribute to decoding calcium signals...
Poraty-Gavra, L., Zimmermann, P., Haigis, S., Bednarek, P., Hazak, O., Stelmakh, O.R., Sadot, E., Schulze-Lefert, P., Gruissem, W., and Yalovsky, S., The Arabidopsis Rho of Plants GTPase AtROP6 Functions in Developmental and Pathogen Response Pathways. Plant Physiology, Vol 161, 2013 (pp. 1172-1188).
How plants coordinate developmental processes and environmental stress...
Bloch, D. and Yalvsky, S. Cell Polarity Signaling. Current Opinion in Plant Biology, Vol 16, 2013, (pp. 734-742).
Cell polarity is a fundamental entity of living organisms...
Kang, E., Zheng, M, Zhang, Y., Yuan, M. Yalovsky, S., Zhu, L. and Fu,Y. The microtubule-associated protein MAP18 affects ROP2 GTPase activity during root hair growth. Plant Physiology, Vol 174, 2017 (pp. 202-222).
Establishment and maintenance of the polar site are important for root hair tip growth...
Sorek, N., Poraty, L., Sternberg, H., Buriakovsky, E., Bar, E., Lewinsohn, E.and Yalovsky, S. Corrected and republished from: Activation Status-Coupled Transient S-Acylation Determines Membrane Partitioning of a Plant Rho-Related GTPase. Molecular and Cellular Biology, Vol 37, 2017 (online: pp. e00333-17).
ROPs or RACs are plant Rho-related GTPases implicated in the regulation...
Feiguelman, G. Fu, Y. and Yalovsky, S. ROP GTPases structure-function and signaling pathways. Plant Physiology, Vol 176, 2018 (pp. 57-79).
Rho of Plants (ROP) proteins, also known as RACs, are the plant-specific subfamily...
Hazak, O., Mamon, E., Lavy, M., Sternberg, H., Behera, S., Schmitz-Thom, I., Bloch, D., Dementiev, O., Gutman, I., Danziger, T., Schwarz, N., Abuzeineh, A., Mockaitis, K. Estelle, M., Hirsch, J. A., Kudla, J. and Yalovsky, S. A novel Ca2+-binding protein that can rapidly transduce auxin responses during root growth. PLoS Biology, Vol 17, 2019, (pp.e3000085).
Signaling cross talks between auxin, a regulator of plant development, and Ca2+...
Bloch, D., Puli, M. R., Mosquna, A. and Yalovsky, S.. Abiotic stress modulates root patterning via ABA-regulated microRNA expression in the endodermis initials. Development, Vol 146, 2019, (pp. dev177097).
Patterning of the root xylem into protoxylem (PX) and metaxylem is regulated by...
Feiguelman, G. and Yalovsky, S. ARMADILLOs delimit Rho signalling. Nature Plants, Vol 6, 2020, (pp. 12012-1202).
Plant development and shape formation is dependent on the establishment...
Hasana Sternberg, Ella Buriakovsky, Daria Bloch, Orit Gutman, Yoav I. Henis and Shaul Yalovsky. Formation of self-organizing functionally distinct Rho of plants domains involves a reduced mobile population. Plant Physiology, Vol 187, 2021 (pp.2485-2508).
Rho family proteins are central to the regulation of cell polarity in eukaryotes. Rho of Plants-Guanyl nucleotide Exchange Factor (ROPGEF) can form self-organizing polar domains following co-expression with an Rho of Plant
Feiguelman G, Cui X, Sternberg H, Ben Hur E, Higa T, Oda Y, Fu Y, & Yalovsky S. (2022) Microtubule-associated ROP interactors affect microtubule dynamics and modulate cell wall patterning and root hair growth. Development, doi: 10.1242/dev.200811
Rho of plant (ROP) proteins and the interactor of constitutively active
ROP (ICR) family member ICR5/MIDD1 have been implicated to
function as signaling modules that regulate metaxylem secondary cell
wall patterning
Xiankui Cui Shuwei Wang, Yaohui Huang, Xuening Ding, Zirong Wang,, Lida Zheng, Yujing Bi, Fanghui Ge, Lei Zhu, MingYuan, Shaul Yalovsky, and Ying Fu. Arabidopsis SYP121 acts as an ROP2 effector in the regulation of root hair tip growth. Molecular Plant, Vol 15, 2022 (pp. 1008-1023). doi: 10.1016/j.molp.2022.04.008.
Tip growth is an extreme form of polarized cell expansion that occurs in all eukaryotic kingdoms to generate highly elongated tubular cells with specialized function
Mallikarjuna R. Pulia, Purity Muchoki, Adi Yaaran, Noga Gershon, Ahan Dalal, Felista Nalufunjo, Yoav Dagan,
Leah Rosental, Shiran Abadi , Zachary Habera , Lucas Silvac, Yariv Brotman, Nir Sade and Shaul Yalovsky
Improving water use efficiency in crops is a significant challenge as it involves balancing
water transpiration and CO2 uptake through stomatal pores.